At the beginning of June 2017 fifty samples of pathological material (internal organs, litter, flushes) of wild waterfowl selected in the territory of the lake were submitted to the Rosselkhoznadzor of the Federal Center for Animal Health. Ubsu-Nur of the Ouyursky district of R. Tyva. All samples were selected and sent by the Office of the Rosselkhoznadzor for R. Khakassia and Tyva in the framework of monitoring activities.
As a result of a complex of laboratory studies, seventeen samples of the internal organs of gulls, cormorants, herons, tern, chomghi and ducks revealed the genome of the avian influenza A virus and the subtype of the detected virus – H5. All work with biological material was carried out in accordance with the recommendations of the OIE for the diagnosis of avian influenza (Manual OIE, 2015).
When carrying out a comparative analysis of the obtained nucleotide sequences of a fragment of the H gene with a length of 258 N, It was found that the isolates under study belong to the Asian genetic line of the highly pathogenic avian influenza virus (line A / Guandong / 1/96, treasure 18.104.22.168). It was established that the sequences of the A viruses of the birds of the subtypes H5N2, H5N6 and H5N8, identified in the territory of the countries of South-East Asia in 2013-2014, proved to be the closest ones for the fragment of the H gene to the isolates identified. (98-100% of coincidences). The cleavage site of hemagglutinin contains basic amino acids and has the structure REKRRKR, which allows us to characterize the virus as potentially highly virulent.
At present, further virological and molecular-biological studies are carried out.
Recent cases of drift of highly pathogenic influenza (AN5N1) by wild birds to the Russian Federation were registered in 2015. On the territory of the Astrakhan and Novosibirsk regions, the Trans-Baikal Territory, R. Tyva. Currently, the disease is prevalent in Southeast Asia, individual countries are endemic over the past few years.
In accordance with Chapter 10.4 of the OIE Code (Terrestrial Animal Health Code, OIE, 2015), the detection of the highly pathogenic influenza virus in wild bird populations does not entail any restrictions in carrying out trade and export operations performed with live domestic (agricultural) poultry or poultry products.
The reference laboratory of viral diseases of birds The FGBI “VNIIZH” draws the attention of the veterinary services of the subjects of the Russian Federation, poultry farms and poultry owners to the inadmissibility of contact of domestic and wild birds, and calls for strict observance of veterinary and sanitary measures for keeping and breeding birds.
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